ACK Lysing Buffer
ACK Lysing Buffer (1X) is a sterile-filtered solution designed for rapid and efficient lysis of erythrocytes while preserving viable leukocytes for downstream laboratory applications. Ideal for preparing peripheral blood, bone marrow, and splenic cell suspensions for flow cytometry, immunology, molecular biology, and cell culture workflows. For Research Use Only.
Description
4Q Life Sciences ACK Lysing Buffer (1X) is a premium, ready-to-use, sterile-filtered solution formulated for the rapid and selective lysis of erythrocytes (red blood cells) while preserving the viability, integrity, and functionality of nucleated cells. Based on the widely established Ammonium-Chloride-Potassium (ACK) formulation, this buffer provides consistent and reproducible red blood cell removal from whole blood and hematopoietic tissue suspensions, making it an essential reagent for research laboratories. Manufactured under controlled conditions using high-purity raw materials and sterile-filtered through a 0.22 μm membrane, 4Q Life Sciences ACK Lysing Buffer is designed to deliver reliable performance across a broad range of cell biology, immunology, molecular biology, and clinical research applications. The optimized formulation effectively lyses erythrocytes while minimizing damage to leukocytes and other nucleated cells, resulting in high-quality cell suspensions suitable for downstream analytical procedures. The buffer is supplied as a 1X ready-to-use solution, eliminating the need for dilution or preparation before use. This reduces preparation time, minimizes operator variability, and improves workflow efficiency in laboratories processing multiple samples. Its room-temperature storage stability further simplifies laboratory inventory management and daily use. ACK Lysing Buffer is commonly used during the preparation of peripheral blood mononuclear cells (PBMCs), leukocytes, splenocytes, bone marrow cells, and other hematopoietic cell suspensions. Following red blood cell removal, samples are suitable for a variety of downstream applications including flow cytometry, immunophenotyping, fluorescence-activated cell sorting (FACS), cell culture, DNA and RNA extraction, PCR, next-generation sequencing, immunological assays, and other molecular and cellular research techniques. Each production batch undergoes rigorous quality control testing to verify appearance, pH, filtration integrity, fill volume, and manufacturing documentation, ensuring consistent performance and high product quality. Batch-specific Certificates of Analysis (COAs) are available through the 4Q Life Sciences product verification system using the product batch number or QR code on the label.
Features
- Ready-to-use 1X formulation
- Sterile filtered (0.22 μm)
- Selective red blood cell lysis
- Preserves leukocyte viability
- High-purity research-grade reagents
- Consistent batch-to-batch performance
- Optimized pH (7.2–7.4)
- Room temperature storage (15–30°C)
- No dilution required
- Suitable for multiple sample types
- Low particulate content
- Quality controlled manufacturing
- Batch traceability
- COA available by batch number
- QR code product verification
- Research Use Only (RUO)
Applications
- Red blood cell lysis
- Leukocyte isolation
- Peripheral blood processing
- Peripheral Blood Mononuclear Cell (PBMC) preparation
- Bone marrow cell preparation
- Splenocyte preparation
- Flow cytometry
- Immunophenotyping
- Fluorescence-Activated Cell Sorting (FACS)
- Cell culture preparation
- DNA extraction workflows
- RNA extraction workflows
- PCR sample preparation
- qPCR sample preparation
- Next-generation sequencing (NGS) sample preparation
- Immunology research
- Cell biology research
- Hematology research
- Molecular biology research
- Biomedical research laboratories
Specifications
| Product Type | Ready-to-Use ACK Lysing Buffer (1X) |
| Formulation | 1X Working Solution |
| Appearance | Clear, Colorless Solution |
| Sterility | Sterile Filtered (0.22 μm) |
| pH | 7.2–7.4 |
| Storage | 15–30°C |
| Shelf Life | 12 Months |
| Container | Sterile HDPE Bottle |
| Volume | 100 mL |
| Quality | Batch Tested |
| COA | Available by Batch Number |
| Intended Use | Research Use Only (RUO) |
| Country of Origin | Uganda |
Store at 15–30°C. Do not freeze.
12 Months
This product is intended for the selective lysis of erythrocytes while preserving nucleated cells for downstream laboratory research applications. It is suitable for use with peripheral blood, bone marrow, spleen, and other hematopoietic tissue samples. For Research Use Only. Not intended for use in diagnostic procedures or therapeutic applications.