Buffers

Dulbecco's Phosphate Buffered Saline, W/O Calcium and Magnessium

Sterile, ready-to-use Dulbecco's Phosphate Buffered Saline (DPBS) 1X formulated without calcium and magnesium for washing cells, sample preparation, reagent dilution, and cell dissociation workflows. Manufactured under strict quality standards to ensure consistent pH, osmolality, and reliable laboratory performance.

Description

4Q Life Sciences Dulbecco's Phosphate Buffered Saline (DPBS) 1X is a sterile, ready-to-use balanced salt solution designed for routine cell culture and molecular biology applications. Formulated without calcium, magnesium, and phenol red, it provides an optimal physiological environment for washing, rinsing, transporting, and diluting mammalian cells while maintaining osmotic balance and stable pH. This formulation is particularly suitable for applications requiring efficient cell dissociation, as the absence of calcium and magnesium minimizes cell adhesion and prevents interference with trypsin or other enzymatic dissociation reagents. It is ideal for washing cells prior to passaging, preparing cells for counting, rinsing cultures before enzymatic treatment, and preparing laboratory reagents. Manufactured under stringent quality-controlled conditions, every batch is sterile-filtered and tested for consistent pH, osmolality, clarity, and sterility. Each bottle is assigned a unique batch number linked to a downloadable Certificate of Analysis (CoA) through the 4Q Life Sciences quality management system, ensuring complete product traceability.

Features

  • Ready-to-use
  • Sterile filtered
  • Research grade quality
  • Calcium & Magnesium free
  • Phenol Red free
  • Physiological pH
  • Optimized osmolality
  • High purity formulation
  • Consistent batch-to-batch performance
  • Quality assured
  • Lot-to-lot traceability
  • Certificate of Analysis (CoA) available
  • QR code product verification
  • Extended shelf life
  • Available in multiple pack sizes

Applications

  • Cell culture
  • Cell washing
  • Cell dissociation
  • Tissue processing
  • Sample preparation
  • Reagent preparation
  • Molecular biology
  • Biotechnology research
  • Immunology
  • Microbiology
  • Flow cytometry
  • Cell counting
  • Diagnostic laboratory workflows
  • Quality control laboratories
  • Academic research
  • Pharmaceutical research
  • Vaccine research
  • Clinical research
  • Life science laboratories
  • Routine laboratory applications

Specifications

For an e-commerce product page, it's better to keep the specifications concise. Here is a curated set of the most important specificationsFor an e-commerce product page, it's better to keep the specifications concise. Here is a curated set of the most important specifications:
**Specifications****Specifications**
Product NameDulbecco's Phosphate Buffered Saline (DPBS) 1X
Brand4Q Life Sciences
GradeCell Culture Grade
FormulationReady-to-use 1X
Calcium & MagnesiumNot present
Phenol RedNot present
SterilitySterile filtered (0.22 µm)
pH7.2–7.4 @ 25°C
Osmolality280–300 mOsm/kg
AppearanceClear, colorless solution
Endotoxin≤1.0 EU/mL
Storage ConditionsStore at 15–30°C. Do not freeze.
Shelf Life24 months
Available Sizes100 mL, 500 mL, 1 L, 5 L, 10 L, 20 L
Quality DocumentationLot-specific Certificate of Analysis (CoA) available
Intended UseFor Research Use Only (RUO)
Manufacturer4Q Life Sciences, a division of Zenotek Scientific Supplies Ltd.
Product Information
SKU4Q-DPBS
TypeBuffer
Concentration1X
Storage

Ambient Temperature

Shelf life

24 Months

Intended use

**Intended Use:** Dulbecco's Phosphate Buffered Saline (DPBS) 1X is intended for washing, rinsing, transporting, and handling mammalian cells and tissues while maintaining physiological pH and osmotic balance. It is suitable for cell culture workflows, cell dissociation, sample preparation, reagent dilution, tissue processing, and other routine laboratory applications. **For Research Use Only (RUO). Not intended for use in diagnostic or therapeutic procedures.**

Pack sizes
500 mL
1000 mL